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Molecular biology
Industry: Biology
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Molecular biology
immunoblotting
Biology; Biochemistry
A technique to detect specific proteins. A mixture of proteins is separated by polyacrylamide-gel electrophoresis, blotted on to a plastic sheet (e.g. of nitrocellulose) and then exposed to a ...
Southern blotting
Biology; Biochemistry
A technique for detection of specific DNA fragments. Restriction fragments are separated by polyacrylamide-gel electrophoresis, blotted on to a plastic sheet (e.g. of nitrocellulose) and detected by ...
Northern blotting
Biology; Biochemistry
By analogy with Southern blotting, a technique for detection of specific RNA molecules; RNA from a cell is denatured and separated by slab gel electrophoresis, then blotted on to a sheet of ...
competitive binding assay
Biology; Biochemistry
A technique for assaying a substance by observing how effective it is, compared with standards, in displacing a fixed amount of the labelled (often radiolabelled) material from a binding site of ...
proofreading
Biology; Biochemistry
In DNA replication, the 3'-to-5' exonuclease activity of a polymerase that removes incorrectly paired bases, also referred to as editing; in protein synthesis, the hydolysis of an incorrectly paired ...
editing
Biology; Biochemistry
In DNA replication, the 3'-to-5' exonuclease activity of a polymerase that removes incorrectly paired bases, also referred to as editing; in protein synthesis, the hydolysis of an incorrectly paired ...
domain
Biology; Biochemistry
A region of a protein that has a stable secondary and tertiary structure that can fold independently of other domains; often connected to other domains, of the same protein if any, by short sequences ...
