Molecular biology

Molecular biology

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Molecular biology

S1 nuclease mapping

Biology; Biochemistry

A technique for identification of nucleotides at the 5'-end of an mRNA which, because it retains at that end its original triphosphate moiety, may be specifically labelled for sequencing by the ...

The suggestion that aggregation of a globular protein occurs when the protein is unfolded, for instance by heat, and the specific intramolecular interactions that determined its native structure are ...

zone of adhesion

Biology; Biochemistry

An area of a lipid bilayer where inner and outer leaflets join, and by which proteins, gangliosides and other products may be targeted to the outer surface of the plasma membrane from their ...

electron tomography

Biology; Biochemistry

A technique for visualizing subcellular structures at very high resolution (near 2.5 nm). The material is prepared for electron microscopy; electron micrographs are taken as the sample is tilted + or ...

magnetic circular dichroism (MCD)

Biology; Biochemistry

A technique to characterize the electronic environment of a metal ion, especially in a metalloprotein. The circular dichroism (CD) spectrum of a sample is recorded in a magnetic field parallel to the ...

nucleomorph

Biology; Biochemistry

A cellular inclusion, bound by multiple membranes, that contains a small amount (ca. 500 kbp) of genomic DNA. These inclusions are thought to be the products of secondary endosymbiosis, e.g. in an ...

cytochrome c

Biology; Biochemistry

A haem protein that is present in the mitochondrial intermembrane space. Its primary function is to act as a single electron relay between respiratory complexes in the respiratory chain, carrying ...