Molecular biology

Molecular biology

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Molecular biology

Cot unit

Biology; Biochemistry

A compound unit used in measurement of the complexity of a DNA preparation. The rate of annealing of DNA is controlled by the second-order rate of nucleation, expressed as C o /C=1+k 2 C o t, ...

A method for fusion of two oligonucleotides, independently of any restriction site they share. A region flanking the desired ligation site on one fragment is introduced into the second fragment by ...

displacement chromatography

Biology; Biochemistry

A column chromatographic method for separation and concentration of components of a solution. After adsorption of the sample to be separated on to the resin in a column, all bound molecules are ...

contact number

Biology; Biochemistry

A measure of the location of an amino acid residue in a protein molecule; the number of α-carbons, excluding those of the adjacent residues in the linear sequence, within an 8 Ã radius of the ...

monodisperse

Biology; Biochemistry

Descriptive of a population of polymers in which all individual molecules have the same covalent structure and molecular size. It is contrasted with polydisperse, descriptive of a population of ...

dialysis

Biology; Biochemistry

A technique for the separation of macromolecules from smaller molecules by placing them within a semi-permeable membrane, such as Cellophane, separating them from a large volume of water. Only the ...

cloverleaf

Biology; Biochemistry

The formalized pattern assumed by a tRNA molecule viewed in two dimensions that shows the regions of internal complementarity that allow the polynucleotide to fold back upon itself into base-paired ...