Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

A sequence of dsDNA that regulates the binding and activity of RNA polymerase. The core promoter includes sequences within 40 nucleotides of a transcription start site; e.g. the initiator, TATA box, ...

node

Biology; Biochemistry

The point of contact of a strand of supercoiled DNA where it loops back on itself. A node, and the writhe of the DNA, may be positive or negative; it is positive if, when viewed so that the strand ...

fluorescence in situ hybridization (FISH)

Biology; Biochemistry

A method for visualization of a genetic marker on a chromosome by use of a fluorescent labeled polynucleotide probe that hybridizes to and indicates the locus of a gene on a chromosome during mitosis ...

vectorette PCR

Biology; Biochemistry

A PCR variation that permits specific amplification of the sequence from a single known internal sequence to the end of the fragment. The source DNA is cleaved by a restriction endonuclease that ...

double-sieve editing

Biology; Biochemistry

A proposed mechanism that accounts for the high selectivity of some enzymes, especially the amino acid activating enzymes. An initial screening sorts substrates by size - those too large cannot enter ...

Monod-Wyman-Changeux (MWC) model

Biology; Biochemistry

A concerted model for the basis of co-operativity in multimeric binding proteins, originally presented as a description of oxygen binding to each of the haem groups of the haemoglobin tetramer. In ...

anchored PCR

Biology; Biochemistry

A variation of the PCR technique, similar to ligation-mediated PCR, that is applied to double-stranded DNA fragments for which the sequence at only one end of the gene is known. The technique allows ...