Molecular biology

Molecular biology

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Molecular biology

loop

Biology; Biochemistry

A packing structure of eukaryotic DNA that may be identical to a replicon. It is seen when the DNA is unfolded and visualized by electron microscopy, and shows the polynucleotide extending from ...

The N-terminal portion of a secretory or membrane protein that assists it across the membrane of the rough endoplasmic reticulum, where it is synthesized, but is cleaved from the protein even before ...

linker-scanner mutation

Biology; Biochemistry

A method to assess the importance of various regions of a protein to its function by introduction of a dipeptide sequence into random sites of the protein. A plasmid that contains a structural gene ...

sulphur cycle

Biology; Biochemistry

The passage of sulphur from organic to inorganic forms, from substituents of cells to minerals, via the following steps: (1) uptake of sulphate from soil by organisms and conversion into organic ...

transposon tagging

Biology; Biochemistry

The use of a transposon to isolate a gene whose product may be unknown. Mutants in which a transposon inserts into and disrupts the function of a gene are selected by phenotype. A DNA fragment that ...

Cotton effect

Biology; Biochemistry

A feature of some optical rotatory dispersion spectra that is usefully correlated with conformational and structural features of a molecule. A sharp peak in optical rotation followed by a deep trough ...

strand displacement amplification (SDA)

Biology; Biochemistry

A technique for amplification of DNA sequences. The source DNA is denatured and hybridized to phosphorothioated primers. HincII nicks its target site on the unmodified strand of the heteroduplex and ...