Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Industry: Biology; Biotechnology

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Biochemistry

Fourier transform-mass spectrometry (FT-MS)

Biology; Biochemistry

A variant of mass spectrometry in which a molecular ion is stabilized in a cyclotron orbit and detected by the frequency of its oscillation. The consequent long half-life and non-destructive ...

In mass spectrometry, an N-terminal fragment of a polypeptide produced by cleavage of the bond between an α-carbon and its neighboring α-carboxy carbon. A b-type ion is an α-N-terminal acylium ion ...

time-of-flight mass spectrometry (TOF-MS)

Biology; Biochemistry

A variant of MS in which the mass of a molecular ion is calculated from the time of travel from the point of ionization to the detector. TOF-MS uses a pulsed ionizing source such as a laser or 252 ...

restriction-site-generating PCR (RG-PCR)

Biology; Biochemistry

A method for detection of a specific mutation that does not itself cause a unique restriction fragment length polymorphism, because it neither creates nor removes a known restriction site. A new ...

mobile barrier

Biology; Biochemistry

A proposed mechanism of membrane transport. A transmembrane protein, the activated porter, exposes its binding site for the transported molecule to one membrane face and then to the other. In the ...

bio-imprinting

Biology; Biochemistry

The induction of a new catalytic specificity in an enzyme in an organic solvent. An enzyme and a weakly binding non-substrate/non-product (e.g. chymotrypsin and N-acetyl-D-tryptophan) are suddenly ...

resonance energy transfer

Biology; Biochemistry

A phenomenon in which one fluorophore excites another when there is significant overlap of the emission spectrum of the primary fluorophore with the excitation spectrum of the secondary fluorophore ...