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Biochemistry
The scientific study of the chemistry of living matter.
Industry: Biology; Biotechnology
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Biochemistry
isomorphous replacement
Biology; Biochemistry
An X-ray crystallographic technique to assist in solving structures from a diffraction pattern. One or more heavy-metal atoms are inserted in the crystal (replacement) without disturbing the ...
heteroduplex
Biology; Biochemistry
A partially double-stranded polynucleotide in which one strand contains sequences not fully complementary to the opposite strand, e.g. mRNA, which contains no introns, annealed to the coding strand ...
imprinting
Biology; Biochemistry
The process by which some genes are rendered non-equivalent. The paternal or maternal allele is not expressed (allelic exclusion), or is expressed differently in different tissues. The phenomenon is ...
Meselson-Radding model
Biology; Biochemistry
A conceptualization of general genetic recombination during meiosis of newly replicated double-stranded DNA. An endonuclease nicks one strand of one daughter duplex; the new 5'-end dissociates and ...
V(D)J recombination
Biology; Biochemistry
The process of assembling the DNA sequences that encode an immunoglobulin or a T-cell receptor (TCR), the cell-surface protein that recognizes an antigen. The process excises polynucleotide sequences ...
Lineweaver-Burk plot
Biology; Biochemistry
A linearized display of kinetic data showing dependence of enzyme activity on substrate concentration, i.e. reciprocal of rate against reciprocal of substrate concentration, therefore also termed a ...
thiocyanate degradation
Biology; Biochemistry
A method for analysis of peptide structure by repetitive sequential degradation from the C-terminus. The free a-carboxyl group is activated with acetic anhydride and reacted with thiocyanate, and the ...
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