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Biochemistry
The scientific study of the chemistry of living matter.
Industry: Biology; Biotechnology
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Biochemistry
hybridoma
Biology; Biochemistry
A cell that is useful in production of quantities of a monoclonal antibody; a fusion of a myeloma cell and a spleen lymphocyte that, like the myeloma cell, can divide in culture and, like the spleen ...
homologous recombination
Biology; Biochemistry
The non-symmetrical crossing-over of genes at homologous sequences (e.g. at Alu sequences of introns between different exons of a single gene) which gives rise to two mutant alleles, one a deletion ...
DNA methylation
Biology; Biochemistry
A phenomenon that represses expression of regions of the genome. Transcription is prevented when the DNA is methylated and folded into nucleosomes. Eukaryotic DNA is methylated almost exclusively as ...
ribosome display
Biology; Biochemistry
An alternative to phage display as a method for selection of a protein with optimized binding properties, which is based on the principles of polysome display. A cell-free expression system ...
polysome display
Biology; Biochemistry
An alternative to phage display as a method for selection of a protein with optimized binding properties. A cell-free bacterial expression system transcribes a DNA library and the resulting mRNAs are ...
pre-initiation complex
Biology; Biochemistry
In replication, transcription or translation, a complex that is potentially composed of template, enzymes and initiation factors but that is incomplete for optimal function due to the absence of one ...
promoter
Biology; Biochemistry
A sequence of dsDNA that regulates the binding and activity of RNA polymerase. The core promoter includes sequences within 40 nucleotides of a transcription start site; e.g. the initiator, TATA box, ...