Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Contributors in Biochemistry

Biochemistry

A difference cloning method; a technique for comparison of two closely related cell types (e.g. a cell with a deletion mutation and the normal cell), and isolation from one (the tester) of DNA that ...

Sanger method (dideoxynucleotide sequencing)

Biology; Biochemistry

A technique for determination of the base sequence of a homogeneous polynucleotide that acts as a template in a replication system. A small amount of a specific dideoxynucleoside triphosphate is ...

cistron

Biology; Biochemistry

A segment of DNA that contains all the information necessary for the production of a single polypeptide and includes both the structural (coding) sequences and regulatory sequences (transcription ...

triplex DNA

Biology; Biochemistry

Triple-stranded DNA in which the third oligonucleotide strand lies in the major groove of duplex DNA. This strand may also be a synthetic oligonucleotide with a sequence designed to target a specific ...

hypoxanthine/aminopterin/thymidine (HAT) medium

Biology; Biochemistry

A growth medium that is used to isolate monoclonal-antibody-producing cells, i.e. hybrids made from fusion of myeloma cell mutants that lack hypoxanthine-guanine phosphoribosyltransferase, and ...

structural profile

Biology; Biochemistry

An approach to prediction of similarities in protein conformations by comparison of amino acid sequences. A two- or three-dimensional plot of one or two features, e.g. optimal matching ...

hyperchromic effect

Biology; Biochemistry

The increase in absorbance, frequently measured at 260nm, when a native polynucleotide is denatured. The ratio of absorbance of the native polymer to that of the denatured or hydrolysed material is ...