Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Industry: Biology; Biotechnology

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Biochemistry

phenol-emulsion reassociation technique (PERT)

Biology; Biochemistry

A method to accelerate several thousand-fold the rate of DNA reassociation. An aqueous solution of single-stranded DNA with a chaotropic salt is shaken with phenol. When the resulting emulsion ...

non-productive

Biology; Biochemistry

In enzymology, descriptive of an enzyme-substrate complex other than the Michaelis complex; one that cannot be converted into an enzyme- product complex. In protein chemistry, descriptive of a ...

The discrepancy between the enormous number of conformations a polypeptide may assume and the rapidity with which it normally achieves its native conformation. It excludes the possibility that each ...

pyranose

Biology; Biochemistry

The form of a sugar when it is condensed into a 6-membered ring. By analogy with pyran, the 6-membered cyclic compound containing an oxygen atom and two conjugated carbon-carbon double bonds, a ...

footprinting

Biology; Biochemistry

A group of techniques for identification of protein-binding sites on DNA. In dimethylsulfate (DMS) footprinting, for example, an end-labelled DNA fragment is partially methylated at guanidine ...

salting out

Biology; Biochemistry

The addition of a salt, especially ammonium sulphate, to decrease the solubility of a susceptible protein. Some proteins respond by becoming more soluble upon addition of a salt, i.e. salting in.

glycobiology

Biology; Biochemistry

The study, at the molecular level, of the structural and functional roles of carbohydrate-containing structures and of the interactions of carbohydrates with other structures, especially proteins.