Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Industry: Biology; Biotechnology

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Biochemistry

Southwestern blotting

Biology; Biochemistry

A technique for identification of DNA-binding proteins and regions of DNA that bind proteins which combines aspects of Southern and western blotting techniques. A nuclear extract is separated by ...

The torsional stress placed on double-stranded DNA, maintained for example by interaction with proteins or by DNA that is circular, and accommodated by a twist imposed on the duplex. A left-handed ...

ion trap

Biology; Biochemistry

A mass spectrograph in which vaporized ions are confined by an AC electric field. As the radio-frequency voltage that confines the ion is changed, the ion will escape the trap at a voltage ...

conjugation

Biology; Biochemistry

In organic chemistry, the interaction of double or triple bonds or aromatic groups, separated by a single bond, which delocalizes their non-bonding electrons; e.g. -CH=CH-CH=CH-, ...

catalytic rate constant (kcat)

Biology; Biochemistry

The first-order rate constant that describes the rate-limiting step in enzyme catalysis, usually the conversion of the enzyme-substrate complex into the enzyme-product complex; the maximal velocity ...

quiescent affinity label

Biology; Biochemistry

A reagent that reacts specifically with the active site of the enzyme for which it was designed. It bears a generally reactive chemical group, such as a chloromethane, and substrate-like moieties ...

magic spot nucleotides

Biology; Biochemistry

Guanine nucleotide derivatives (ppGpp and pppGpp) that mediate the cessation of production of rRNA in the absence of an essential amino acid, a phenomenon present in stringent bacteria but absent ...