Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Industry: Biology; Biotechnology

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Biochemistry

yeast artificial chromosome (YAC)

Biology; Biochemistry

A cloning vector with a yeast that can accept a relatively large fragment of foreign DNA, up to 1 Mb, in yeast cells; a YAC has telomers on each end and a centromere. Their usefulness is limited by ...

PCR amplification of multiple specific alleles (PAMSA)

Biology; Biochemistry

A method for determination of zygosity. Three primers are used, two of which are allele-specific and one of which matches the complementary strand of both alleles. Allele specificity is achieved by ...

biosensor

Biology; Biochemistry

A device, especially an electrochemical device that detects, quantitatively and in real time, the presence of an anylate or some biological event (e.g. respiration, enzymic activity, binding to an ...

PCR amplification of specific alleles (PASA)

Biology; Biochemistry

PASA; a method for genotyping of single-base mutations. Genomic DNA is used as a template in two reactions, one with a primer that makes a perfect match to the wild-type allele, another with a primer ...

sticky ended

Biology; Biochemistry

Descriptive of the structure of a double-stranded polynucleotide in which one strand extends further from the end than the other to create a single-stranded 'tail'; especially the product of cleavage ...

inclusion body

Biology; Biochemistry

An amorphous deposit in the cytoplasm of a cell; an aggregated protein appropriate to the cell but damaged, improperly folded or liganded, or a similarly inappropriately processed foreign protein, ...

framework model

Biology; Biochemistry

A proposed sequence of protein folding events in which the random coil, or denatured form, passes through three stages: the formation of fluctuating embyros, in which disconnected regions of ...