Molecular biology

Molecular biology

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Molecular biology

pool

Biology; Biochemistry

The aggregate of metabolites that are in a functional equilibrium in a cell or an organism, e.g. cytosolic hexose phosphates, mitochondrial tricarboxylic acid cycle intermediates, liver glycogen.

reverse transcriptase-PCR (RT-PCR)

Biology; Biochemistry

Also known as RNA phenotyping, RNA-PCR or message amplification phenotyping; a method for amplification of a specific mRNA by prior use of reverse transcriptase to form a cDNA, then use of PCR to ...

An indication of the position of an amino acid residue within a proposed folding of a globular protein; the number of α-carbons within a 14Ã (1.4nm) radius, which varies from about 10 for a residue ...

nuclear Overhauser and exchange spectroscopy (NOESY)

Biology; Biochemistry

A nuclear magnetic resonance method for evaluation of internuclear distances (within 4Å (0.4nm)) between non-covalently bonded nuclei; one center is saturated with radiation and, as its excited state ...

N-terminal

Biology; Biochemistry

In a polypeptide sequence, that unique residue which is connected to the linear sequence by its carboxy group, leaving it with a free amino group. In practice, the amino group of an N-terminal ...

nicking

Biology; Biochemistry

As contrasted with cutting, the cleaving of a single, or very few, phosphodiester bonds in one polynucleotide strand of a DNA duplex; also the cleavage of a peptide bond in a protein that does not ...

genomic SELEX

Biology; Biochemistry

A variant of the SELEX (systematic enrichment of ligands by experimental enrichment) procedure, that uses protein ligands and genomic DNA fragments from the same organism to select RNA-protein ...