Molecular biology

Molecular biology

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Molecular biology

RNA interference (RNAi)

Biology; Biochemistry

The phenomenon of gene silencing effected by the interaction of a double-stranded RNA, approx. 22 bp long, with the cognate genomic DNA sequence. The RNA-DNA complex is subject to hydrolysis by a ...

kinesin

Biology; Biochemistry

A family of motor proteins that binds to microtubules and then translocates toward the plus end. They typically also bind to cargo such as intracellular vesicles, so that motor activity moves the ...

A flexible term applied to the details of a process that links initial and final states, e.g. the mechanism of respiration, the mechanism of fatty acid oxidation, the mechanism of β-oxoacyl-coenzyme ...

enhancer

Biology; Biochemistry

A 50 to 1500 bp dsDNA segment that up-regulates transcription of a gene. The enhancer may be up- or downstream or even within an intron of the gene. It is distinct from the promoter; its action may ...

host-guest protocol

Biology; Biochemistry

An experiment designed to evaluate the contribution of different amino acids at a single sequence position to the stability of a polypeptide. Site-directed mutagenesis or synthetic chemical ...

intron homing

Biology; Biochemistry

The direction of introns, as mobile genetic elements, to sites in DNA. The process is dependent upon proteins that are encoded by open reading frames of the introns: endonucleases in the case of ...

equilibrium labelling

Biology; Biochemistry

In metabolic studies, the exposure of a biological system (e.g. a tissue slice, a cell culture, mitochondria) to a radiolabelled metabolite over a relatively long time so as to establish a constant ...