Molecular biology

Molecular biology

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Molecular biology

rolling circle replication

Biology; Biochemistry

Mechanisms of DNA replication of small circular genomes, such as some viruses and plasmids, in which the template generates multiple tandem copies. One strand is nicked and serves as a primer for ...

topology/packing diagram

Biology; Biochemistry

A schematic representation of secondary structure of a protein in which α-helix is rendered as a circle, β-sheet as a rectangle and connecting sequences as lines. The circles and rectangles are ...

nuclear run-off assay

Biology; Biochemistry

A method to determine which genes in a population of cells are expressed at a given time. Nascent RNA transcripts are radiolabelled with ( 32 P)NTPs as they are elongated in isolated nuclei (new ...

The form of energy that is capable of doing work at constant pressure and temperature; a concept that bridges the requirement that energy (enthalpy plus potential energy) be conserved and that the ...

equilibrium sedimentation ultracentrifugation

Biology; Biochemistry

A technique for evaluation of the molecular mass of a polymer by determination of the extent of sedimentation at which the force exerted upon a macromolecule in a centrifuge is balanced by its ...

branch site

Biology; Biochemistry

In RNA splicing, the site to which the 5'-guanylate at the end of an intron is joined to an adenylate residue within the intron through a 2',5'-phosphodiester bond to form a 'lariat' intermediate.

sequence-tagged connector (STC)

Biology; Biochemistry

An approximately 500 bp sequence at each end of a bacterial artificial chromosome (BAC). If the human genome is cleaved into 300000 fragments, of an average length of 150 kb, there would be 600000 ...