Biochemistry

Biochemistry

The scientific study of the chemistry of living matter.

Contributors in Biochemistry

Biochemistry

A technique for detection of specific DNA fragments. Restriction fragments are separated by polyacrylamide-gel electrophoresis, blotted on to a plastic sheet (e.g. of nitrocellulose) and detected by ...

Northern blotting

Biology; Biochemistry

By analogy with Southern blotting, a technique for detection of specific RNA molecules; RNA from a cell is denatured and separated by slab gel electrophoresis, then blotted on to a sheet of ...

competitive binding assay

Biology; Biochemistry

A technique for assaying a substance by observing how effective it is, compared with standards, in displacing a fixed amount of the labelled (often radiolabelled) material from a binding site of ...

proofreading

Biology; Biochemistry

In DNA replication, the 3'-to-5' exonuclease activity of a polymerase that removes incorrectly paired bases, also referred to as editing; in protein synthesis, the hydolysis of an incorrectly paired ...

editing

Biology; Biochemistry

domain

Biology; Biochemistry

A region of a protein that has a stable secondary and tertiary structure that can fold independently of other domains; often connected to other domains, of the same protein if any, by short sequences ...

comparative anchor tagged site (CATS)

Biology; Biochemistry

An extended DNA sequence that is sufficiently conserved across a spectrum of species, e.g. all mammals or mouse and human, so as to serve as a marker for comparative genome mapping and assist the ...