Molecular biology

Molecular biology

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Molecular biology

Hunter and Downs plot

Biology; Biochemistry

A graphical method for determination of dissociation constants of enzyme inhibitors. Where I is total inhibitor concentration and α is the ratio of inhibited to non-inhibited rates, a plot of ...

A graphical method for determination of enzyme-inhibitor dissociation constants for non-competitive inhibitors, especially those that bind very tightly. Where I is the total inhibitor concentration, ...

Dixon plot

Biology; Biochemistry

A graphical method for determination of the type of enzyme inhibition and the dissociation constant (K i ) for an enzyme-inhibitor complex. The effect on the enzymic rate (v) is determined at two or ...

complexity

Biology; Biochemistry

In nucleic acid chemistry, a measure of the unique sequences in a DNA preparation; it increases with chain length and decreases with the extent to which the sequences are repetitive; evaluated as the ...

fluorescence-activated cell sorting (FACS)

Biology; Biochemistry

A technique for separation of cells according to their fluorescence after attachment of a fluorophore to specific cells, e.g. with a fluorescent antibody. Droplets that contain no more than one cell ...

Gortner and Grendel model

Biology; Biochemistry

A model in which erythrocyte membranes comprise only phosphoacylglycerols, i.e. no protein or sterol component is envisaged; the model presents a bilayer in which each leaflet is composed of lipids ...

apoptosis

Biology; Biochemistry

A morphologically characterized process of programmed cell-death, initiated by various physiological or pathological causes (e.g. cell turnover, hormone-induced atrophy, cell-mediated immune ...