Molecular biology

Molecular biology

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Molecular biology

cross-reacting material

Biology; Biochemistry

A protein product resulting from mutation that has lost its function but is recognizable by its ability to react with antibodies raised against the normal protein. More broadly, material may ...

A protocol for generation of single-stranded DNA. Unequal amounts of primers are used, so that the first PCR cycles generate equal amounts of each strand of the template but later cycles, which have ...

nucleotide-binding domain

Biology; Biochemistry

One of the homologous supersecondary structures of some proteins, composed of Rossmann folds, to which may be bound the AMP moiety of a substrate or ligand; e.g. the domain of a dehydrogenase that ...

retention of configuration

Biology; Biochemistry

The lack of a change in the configuration at an asymmetrical center over the course of a reaction, e.g. the enzymic addition of an α-glucosyl unit to glycogen from UDP-glucose, which is itself an ...

post-transcriptional modification

Biology; Biochemistry

The processing of RNA subsequent to its synthesis. May include hydrolysis and transesterification of phosphodiester bonds and modification of bases. For eukaryotic mRNA it includes capping of the ...

troponin

Biology; Biochemistry

A complex of three proteins (TnI, TnC and TnT) that interacts with tropomyosin to control cross bridge formation during skeletal and cardiac muscle contraction. Troponin concentrations are elevated ...

electron density map

Biology; Biochemistry

In X-ray crystallography, a representation that resembles a geological survey map of planes drawn through a crystal. It shows contours of equal electron density surrounding the individual atoms: ...