Molecular biology

Molecular biology

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Molecular biology

RNA virus

Biology; Biochemistry

A virus with an RNA genome that may be either an mRNA, (+)-RNA, or its complement, (-)-RNA. Class 1 contains (+)-RNA; class 2, (-)-RNA, which is the template for an RNA-dependent RNA polymerase; ...

superelectrophile

Biology; Biochemistry

A chemical species with higher than usual electrophilic reactivity, often involving two positively charged centers. Superelectrophiles are intermediates in some enzymatic reactions; e.g. those of ...

Also known as scanning force microscopy; a method for mapping the surface of microscopic (e.g. cellular) and even submicroscopic (e.g. macromolecular) surfaces. As a sharp tip passes over the surface ...

Dintzis experiment

Biology; Biochemistry

The method by which it was demonstrated that proteins are synthesized in the N- to C-terminal direction. A labelled amino acid was supplied to a reticulocyte preparation synthesizing haemoglobin and ...

trans-recognition

Biology; Biochemistry

A refinement of the N-end rule; the determination of the rate of degradation of a cellular multimeric protein composed of different kinds of subunits by amino acid residues of a subunit other than ...

somatic cell nuclear transfer (SCNT)

Biology; Biochemistry

An experimental technique for cloning an organism. Potentially, the technique can be adapted to growing an exactly compatable tissue or organ for transplantation, and would then be called therapeutic ...

PCR-based differential scanning

Biology; Biochemistry

A method for amplification of a heterogeneous population of cDNAs preparatory to creation of a library. Total mRNA is copied first using an oligo(dT) primer to obtain one copy, then that primer is ...