Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

A technique for protein separation that uses high-pressure liquid chromatography followed by sodium dodecyl sulphate (SDS)/polyacrylamide-gel electrophoresis. The effluent from a reverse-phase column ...

C-terminus

Biology; Biochemistry

The carboxyl end of a protein. As proteins are linear polymers of α-amino acids H 2 N-CHR a -CO-(NH-CHRÂ-CO) n -NH-CHR z -COOH where R is one of the 20 chemical groups that distinguish the amino ...

origin recognition complex (ORC)

Biology; Biochemistry

An aggregate of nuclear proteins involved in initiation of replication and/or repression of transcription (silencing). The DNA regions that bind the ORC are examples of anomalously replicating ...

delta restriction cloning

Biology; Biochemistry

A method for the use of a single primer to determine a DNA sequence longer than would normally be practical for a single sequencing attempt. After the first sequencing the bulk of the determined ...

C-terminal analysis

Biology; Biochemistry

A chemical method for determination of C-terminal residues of proteins by cleavage by hydrazinolysis, which generates amino acid hydrazides of all amino acids except the C-terminal one; also an ...

carboxyl-terminal analysis

Biology; Biochemistry

A chemical method for determination of C-terminal residues of proteins by cleavage by hydrazinolysis, which generates amino acid hydrazides of all amino acids except the C-terminal one; also an ...

residualizing label

Biology; Biochemistry

A moiety attached to a circulating protein in order to subsequently detect by histological means the site of the protein's degradation in a multicellular organism. A reporter group, such as a ...