Molecular biology

Molecular biology

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Molecular biology

quantitative PCR (QPCR)

Biology; Biochemistry

A method for quantification of a polynucleotide by inclusion with it of a known amount of an easily distinguished template as an internal standard to compensate for variation in efficiency of ...

The unique pattern of ninhydrin-reactive spots found upon separation of a tryptic digest of a protein by paper electrophoresis in one dimension, followed by chromatography in a second dimension at a ...

feedback inhibition

Biology; Biochemistry

A form of metabolic control in which the end product of a pathway inhibits the enzyme, usually an allosteric enzyme, that catalyzes the earliest irreversible reaction that is unique to the pathway. ...

molecular dynamics

Biology; Biochemistry

The computer-simulated quantitative evaluation of a molecular model by assignment of force constants to bonded and non-bonded interactions between neighboring atoms so that bond distances and angles ...

alte gelbe ferment

Biology; Biochemistry

The name given to what has become known as the flavoprotein NAPDH dehydrogenase, after a second flavoprotein was discovered. The translated name, old yellow enzyme or OYE, is still current. The ...

transpeptidation

Biology; Biochemistry

A characteristic of some proteinases; the transfer of one product of the catalytic cleavage to another peptide rather than to water; the transfer of the N-terminal fragment to the amino group of an ...

genetic dissection

Biology; Biochemistry

An approach to defining the roles of individual factors in a complex system. Mutations in a metabolic pathway or physiological function are selected and the function of the cognate gene product is ...