Molecular biology

Molecular biology

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Molecular biology

sucrose-gradient centrifugation

Biology; Biochemistry

A technique for characterization or preparation of subcellular particles. A swinging-bucket-type centrifuge tube is filled with a sucrose gradient, the bottom of which is most dense and the top least ...

van den Bergh reaction

Biology; Biochemistry

A method for colorimetric estimation of serum bilirubin by coupling with diazotized sulphanilic acid. The direct van den Bergh reaction, in aqueous medium, yields the 'direct' bilirubin, i.e. the ...

sensitivity

Biology; Biochemistry

In quantitative terms, the responsiveness of a physiological system to a stimulus, such as an allosteric effector, substrate or hormone. Where X is the magnitude of the stimulus and Y the magnitude ...

enzymic inverse PCR (EI-PCR)

Biology; Biochemistry

A method for site-directed mutagenesis which generates highly efficient plasmids and which uses only two PCR primers. The primers incorporate the same site for a restriction nuclease, the recognition ...

phylogenetic tree

Biology; Biochemistry

A reconstruction of the evolutionary relationship of contemporary species that shows their divergence from common ancestors and the branch points at which different species separated. A common ...

evolutionary tree

Biology; Biochemistry

A reconstruction of the evolutionary relationship of contemporary species that shows their divergence from common ancestors and the branch points at which different species separated. A common ...

shotgun sequencing

Biology; Biochemistry

An approach to determination of the sequence of a large, randomly selected, piece of genomic DNA. A cosmid that contains the DNA is sonicated into smaller fragments of 1.0-1.5kb, which are ligated ...