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Molecular biology
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Molecular biology
zipper-type mechanism
Biology; Biochemistry
A mechanism for proteolytic degradation of a native protein, in which cleavage of the first peptide bond in each molecule is much faster than subsequent cleavages. The result is that every native ...
indirect end labelling
Biology; Biochemistry
A method to locate, between restriction sites, binding sites on DNA, e.g. the positions of nucleosomes on a minichromosome. The DNA protected by binding proteins is cleaved in susceptible, i.e. ...
caveolae
Biology; Biochemistry
Small invaginations of the plasma membrane, characterized by glypiated proteins among others, that provide binding sites and points of entry of small molecules into the cytoplasm via trancytosis or ...
quinimine form
Biology; Biochemistry
A tautomer of the Schiff base adduct of pyridoxal phosphate with an amino acid in which the pyridine ring nitrogen is protonated but uncharged and the carbon atom of the coenzyme that attaches the ...
RNA ligase reverse transcription PCR (RL-RT-PCR)
Biology; Biochemistry
A procedure for amplification of minute amounts of an unknown RNA (or DNA) sequence. The RNA fragment is ligated at both ends to single-stranded palindromic oligodeoxynucleotide that provides a site ...
bacteriophage
Biology; Biochemistry
A virus that infects bacteria. Many phage have proved useful in the study of molecular biology and as vectors for the transfer of genetic information between cells. Lytic phage, e.g. the T series ...
hemisubstrate
Biology; Biochemistry
An enzyme inhibitor that acts in the initial phases of enzyme action as a substrate, but does not complete the catalytic cycle to regenerate an active enzyme; e.g. organophosphate ester ...