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Molecular biology

Contributors in Molecular biology

Molecular biology

co-metabolism

Biology; Biochemistry

In microbial physiology, the metabolism of a xenobiotic which does not support growth. The phenomenon possibly involves the fortuitous metabolism of material due to partial non-specificity of key ...

nested primers

Biology; Biochemistry

A second set of PCR primers whose use follows an initial copying of a nucleic acid sequence; the nested primers are specific for sequences internal to the 3'- and 5'-ends of the originally copied ...

acceptor (3') splice site

Biology; Biochemistry

A site in pre-mRNA that corresponds to the 3'-end of the intron and the 5'-end of the next exon. The last two bases of the intron at the acceptor splice site are AG in most cases, but AC in some ...

cloning of ligand targets (COLT)

Biology; Biochemistry

A strategy for screening phage cDNA libraries for genes that encode proteins that can bind a desired ligand. Phage which express the protein are identified on plates the ability to bind the labeled ...

stress-fibre

Biology; Biochemistry

An actomyosin-containing filament of a non-muscle cell. Between mitoses (interphase) the fibres are anchored at membrane attachment sites and are involved in extension of lamellipodia and in cell ...

gene therapy

Biology; Biochemistry

The in vivo introduction of a functional gene, and its expression, in the germ line cells (germ line therapy) or somatic cells (somatic gene therapy) of an individual who does not possess the normal ...

vaccinome

Biology; Biochemistry

A plasmid that contains a DNA sequence which encodes an immunologically effective protein, such as an HLA T-cell epitope. A string-of-beads vaccine plasmid is a vaccinome that contains many such ...

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