Molecular biology

Molecular biology

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Molecular biology

perfusion chromatography

Biology; Biochemistry

A variant of high-pressure liquid chromatography in which particles as the stationary phase have pores large enough (6000–8000 Ã (600–800 nm)) to permit solvent flow-through and thus allow much ...

A mechanism for transport of metabolites or chemical groups across the mitochondrial membrane, e.g. the electron shuttle that transports glycerophosphate into, and dihydroxyacetone phosphate out of, ...

Caenorhabitis elegans

Biology; Biochemistry

A nematode; a small worm favored by developmental and molecular biologists because if its ability to grow under laboratory conditions, its short generation time and its transparency. Because of its ...

hydrazinolysis

Biology; Biochemistry

In determination of the C-terminal amino acid of a protein, cleavage of a peptide bond by insertion across it of the elements of hydrazine; in the Maxam-Gilbert method for sequencing polynucleotides, ...

bile acid

Biology; Biochemistry

One of the products of cholesterol hydroxylation and side-chain oxidation to the level of a carboxylic acid. The carboxylate is often conjugated through an amide bond to a glycine or a cysteic acid. ...

sigmoidal

Biology; Biochemistry

S-shaped; descriptive of the appearance of a graph that shows dependence of rate on substrate concentration for a multimeric enzyme that is accelerated by binding of more than one substrate molecule; ...

apparent Km

Biology; Biochemistry

The Michaelis constant as observed under conditions (e.g. the presence of a competitive inhibitor) that would hinder the determination of its true value; in the case of a two-substrate enzyme, the ...