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Molecular biology
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Molecular biology
marker rescue
Biology; Biochemistry
A technique to localize the site of a mutation within a gene. A polynucleotide, the 'marker', from the known wild-type sequence is allowed to recombine with the mutant genome such that, if it ...
vector
Biology; Biochemistry
An animal host and carrier of a pathogen; e.g. the flea which transmits the bacterium Pasteurella pestis; also, a DNA molecule that can be replicated in a cell and that can serve as the vehicle for ...
deletion map
Biology; Biochemistry
The linear arrangement of natural mutations along a chromosome or part of a chromosome. In the first phase many individuals are studied for the presence of cytogenetic markers; later phases use ...
beta (β)-ribbon
Biology; Biochemistry
A DNA-recognition motif; a segment of antiparallel β-structure that can fit into the major groove of a double-stranded polynucleotide and bind to it due to very specific interactions between side ...
depletion-insertion
Biology; Biochemistry
A technique used to define properties of a cellular, subcellular or tissue model, in which a variable or undesired constituent, e.g. membrane phospholipid, is removed (by a phospholipase) and then ...
mating-type switching
Biology; Biochemistry
A phenomenon in the behavior of yeast and other organisms that rapidly change from the haploid to the diploid state. In yeast the two mating types are a and α; when two a or two α cells mate, the ...
melting temperature
Biology; Biochemistry
In nucleic acid and protein chemistry, and by analogy with organic chemistry, the temperature that is the mid-point for the thermal unfolding of a nucleic acid or protein; in the case of nucleic ...
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