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Molecular biology

Contributors in Molecular biology

Molecular biology

manometry

Biology; Biochemistry

A largely obsolete technique for measurement of respiration of tissue slices or other biochemical preparations. The volume of oxygen taken up is measured in a Warburg apparatus, a closed system that ...

rapid amplification of cDNA ends (RACE)

Biology; Biochemistry

A PCR-based method to obtain cDNA clones of rare transcripts which begin with only a partial internal sequence. The 3'- and 5'-ends are analyzed separately; a single (-)-strand is first constructed ...

coated region

Biology; Biochemistry

The region of the plasma membrane of a cell capable of endocytosis that contains receptors and is lined on the cytoplasmic side with the protein clathrin. When the receptors are occupied, these ...

Maxam-Gilbert method

Biology; Biochemistry

A technique for sequence analysis of DNA in which four chemical reactions are applied separately, each to cleave the polynucleotide randomly at one of the four bases. Subsequent polyacrylamide-gel ...

chemical cleavage method

Biology; Biochemistry

A technique for sequence analysis of DNA in which four chemical reactions are applied separately, each to cleave the polynucleotide randomly at one of the four bases. Subsequent polyacrylamide-gel ...

instructive theory

Biology; Biochemistry

A now discarded hypothesis that explains antibody specificity by the folding of an antibody structure, which has the potential for many specificities, to the unique surface of a particular antigen.

chip

Biology; Biochemistry

In biotechnology, a component of a device for screening genomic or cDNA for mutations, polymorphisms or gene expression. A chip is a small (a few centimeters on each side) standardized glass or other ...

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