15 Terms
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Molecular biology
Industry: Biology
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Molecular biology
oligonucleotide array
Biology; Biochemistry
In biotechnology, a component of a device for screening genomic or cDNA for mutations, polymorphisms or gene expression. A chip is a small (a few centimeters on each side) standardized glass or other ...
Southwestern blotting
Biology; Biochemistry
A technique for identification of DNA-binding proteins and regions of DNA that bind proteins which combines aspects of Southern and western blotting techniques. A nuclear extract is separated by ...
supercoiling
Biology; Biochemistry
The torsional stress placed on double-stranded DNA, maintained for example by interaction with proteins or by DNA that is circular, and accommodated by a twist imposed on the duplex. A left-handed ...
ion trap
Biology; Biochemistry
A mass spectrograph in which vaporized ions are confined by an AC electric field. As the radio-frequency voltage that confines the ion is changed, the ion will escape the trap at a voltage ...
conjugation
Biology; Biochemistry
In organic chemistry, the interaction of double or triple bonds or aromatic groups, separated by a single bond, which delocalizes their non-bonding electrons; e.g. -CH=CH-CH=CH-, ...
catalytic rate constant (kcat)
Biology; Biochemistry
The first-order rate constant that describes the rate-limiting step in enzyme catalysis, usually the conversion of the enzyme-substrate complex into the enzyme-product complex; the maximal velocity ...
quiescent affinity label
Biology; Biochemistry
A reagent that reacts specifically with the active site of the enzyme for which it was designed. It bears a generally reactive chemical group, such as a chloromethane, and substrate-like moieties ...