Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

In biotechnology, a component of a device for screening genomic or cDNA for mutations, polymorphisms or gene expression. A chip is a small (a few centimeters on each side) standardized glass or other ...

Southwestern blotting

Biology; Biochemistry

A technique for identification of DNA-binding proteins and regions of DNA that bind proteins which combines aspects of Southern and western blotting techniques. A nuclear extract is separated by ...

supercoiling

Biology; Biochemistry

The torsional stress placed on double-stranded DNA, maintained for example by interaction with proteins or by DNA that is circular, and accommodated by a twist imposed on the duplex. A left-handed ...

ion trap

Biology; Biochemistry

A mass spectrograph in which vaporized ions are confined by an AC electric field. As the radio-frequency voltage that confines the ion is changed, the ion will escape the trap at a voltage ...

conjugation

Biology; Biochemistry

In organic chemistry, the interaction of double or triple bonds or aromatic groups, separated by a single bond, which delocalizes their non-bonding electrons; e.g. -CH=CH-CH=CH-, ...

catalytic rate constant (kcat)

Biology; Biochemistry

The first-order rate constant that describes the rate-limiting step in enzyme catalysis, usually the conversion of the enzyme-substrate complex into the enzyme-product complex; the maximal velocity ...

quiescent affinity label

Biology; Biochemistry

A reagent that reacts specifically with the active site of the enzyme for which it was designed. It bears a generally reactive chemical group, such as a chloromethane, and substrate-like moieties ...