Molecular biology

Molecular biology

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Molecular biology

pulse labelling

Biology; Biochemistry

In metabolic studies, the exposure of a biological system (e.g. a tissue slice, a cell culture, mitochondria) to a radiolabelled metabolite over a relatively short time, in order to subsequently ...

sedimentation-velocity ultracentrifugation

A technique that measures the rate of movement of a dissolved polymer under the force exerted upon it in a centrifuge, to assist in evaluation of molecular mass, axial ratio and other hydrodynamic ...

scintillation counting

Biology; Biochemistry

A technique for detection and quantification of radioactivity by capture of the emission from a radiolabel by a primary phosphor which, on emission, excites a secondary phosphor and causes it to emit ...

endocytosis

Biology; Biochemistry

A process by which extracellular matter is taken up by a cell; the plasma membrane invaginates and encloses the material in newly formed vesicles. In phagocytosis the extracellular material is ...

Fc fragment

Biology; Biochemistry

A product of papain hydrolysis of an immunoglobulin G consisting of parts of the constant regions of two heavy chains held together by a disulphide bridge, but excluding antigen-binding regions.

fast-atom bombardment mass spectrometry (FAB-MS)

Biology; Biochemistry

A technique for the analysis of protein sequence and structure. A high energy beam of atoms or ions (Cs or Xe) vapourizes, fragments and ionizes a protein solution, the mass spectrum of which gives ...

fast-atom bombardment (FAB) mapping

Biology; Biochemistry

A technique for comparison of recombinant with natural proteins or with expected structures. A protein is cleaved by a specific enzymic (e.g. trypsin) or chemical (e.g. CNBr) method and the product ...