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Molecular biology
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Molecular biology
infrared spectroscopy
Biology; Biochemistry
IR spectroscopy; a technique that measures absorption of electromagnetic radiation between the long wavelength end of the visible spectrum (780‒800 nm) and the short end of the microwave spectrum ...
phenol-emulsion reassociation technique (PERT)
Biology; Biochemistry
A method to accelerate several thousand-fold the rate of DNA reassociation. An aqueous solution of single-stranded DNA with a chaotropic salt is shaken with phenol. When the resulting emulsion ...
non-productive
Biology; Biochemistry
In enzymology, descriptive of an enzyme-substrate complex other than the Michaelis complex; one that cannot be converted into an enzyme- product complex. In protein chemistry, descriptive of a ...
paradox of Levinthal
Biology; Biochemistry
The discrepancy between the enormous number of conformations a polypeptide may assume and the rapidity with which it normally achieves its native conformation. It excludes the possibility that each ...
pyranose
Biology; Biochemistry
The form of a sugar when it is condensed into a 6-membered ring. By analogy with pyran, the 6-membered cyclic compound containing an oxygen atom and two conjugated carbon-carbon double bonds, a ...
footprinting
Biology; Biochemistry
A group of techniques for identification of protein-binding sites on DNA. In dimethylsulfate (DMS) footprinting, for example, an end-labelled DNA fragment is partially methylated at guanidine ...
salting out
Biology; Biochemistry
The addition of a salt, especially ammonium sulphate, to decrease the solubility of a susceptible protein. Some proteins respond by becoming more soluble upon addition of a salt, i.e. salting in.
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