Molecular biology

Molecular biology

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Molecular biology

analytical ultracentrifugation

Biology; Biochemistry

A technique of very-high-speed centrifugation that sediments soluble macromolecules and characterizes them according to their rate of sedimentation (sedimentation-velocity ultracentrifugation) or the ...

isomorphous replacement

Biology; Biochemistry

An X-ray crystallographic technique to assist in solving structures from a diffraction pattern. One or more heavy-metal atoms are inserted in the crystal (replacement) without disturbing the ...

heteroduplex

Biology; Biochemistry

A partially double-stranded polynucleotide in which one strand contains sequences not fully complementary to the opposite strand, e.g. mRNA, which contains no introns, annealed to the coding strand ...

imprinting

Biology; Biochemistry

The process by which some genes are rendered non-equivalent. The paternal or maternal allele is not expressed (allelic exclusion), or is expressed differently in different tissues. The phenomenon is ...

Meselson-Radding model

Biology; Biochemistry

A conceptualization of general genetic recombination during meiosis of newly replicated double-stranded DNA. An endonuclease nicks one strand of one daughter duplex; the new 5'-end dissociates and ...

V(D)J recombination

Biology; Biochemistry

The process of assembling the DNA sequences that encode an immunoglobulin or a T-cell receptor (TCR), the cell-surface protein that recognizes an antigen. The process excises polynucleotide sequences ...

Lineweaver-Burk plot

Biology; Biochemistry

A linearized display of kinetic data showing dependence of enzyme activity on substrate concentration, i.e. reciprocal of rate against reciprocal of substrate concentration, therefore also termed a ...