Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

A method for analysis of peptide structure by repetitive sequential degradation from the C-terminus. The free a-carboxyl group is activated with acetic anhydride and reacted with thiocyanate, and the ...

hydrophobic moment

Biology; Biochemistry

A measure of the amphipathicity of an Â¿-helix. Each amino acid side chain is assigned a value, positive or negative, according to its hydrophobicity. These values are used to weight vectors for each ...

electroblotting

Biology; Biochemistry

An electrophoretic technique for transfer of a protein or nucleic acid from a slab gel, or similar unstable two-dimensional matrix, to a sheet of stable material such as nitrocellulose or ...

radioimmunoassay (RIA)

Biology; Biochemistry

A competitive binding assay that depends upon displacement of a radiolabelled ligand from an antibody by the non-labelled standard or test sample, followed by separation of the labelled ligand into ...

glycolysis

Biology; Biochemistry

One of the central pathways of metabolism in most eukaryotes and many other cells; the sequence of enzymic reactions that converts glucose into lactic acid (anaerobic glycolysis) or into pyruvate ...

kinetic assay

Biology; Biochemistry

An enzyme-based assay that measures the amount of substrate present by correlation of the rate of reaction with the known dependence of the rate on substrate concentration, usually under first-order ...

endorphin

Biology; Biochemistry

Endogenous morphine-like peptide; one of a class of peptides that are derived from pro-opiomelanocortin by limited proteolysis and have analgaesic properties. The group includes the 31-residue ...