Molecular biology

Molecular biology

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Molecular biology

microarray separation

Biology; Biochemistry

A method for sorting uniformly charged macromolecules, particularly nucleic acids, by size. Unlike conventional electrophoretic separations, the method uses a gel phase. By bonding a flat silicon ...

The necessary sequence of growth and synthetic stages through which a cell passes, from its origin by mitosis of a parent cell until its own division into daughter cells. It consists of a G 1 (first ...

proteasome

Biology; Biochemistry

A multicatalytic proteinase; a molecular organelle of size up to 26 S (2000 KDa) in eukaryotes. The basic 20 S (700 KDa) structure is a barrel-like structure composed of two inner rings, each of 7 ...

real time quantitative PCR

Biology; Biochemistry

A method for quantification of nucleic acid sequences by PCR amplification in the presence of doubly-labelled probe. The 5'-end contains a reporter fluorophore, e.g. carboxyfluorescein, and the ...

ion-channel switch (ICS)

Biology; Biochemistry

A biosensor that depends upon the alignment of ion half-channels embedded in the immobile inner and the fluid outer leaflets of a lipid bilayer to permit the flux of ions (e.g. monovalent cations ...

nucleic acid sequence-based amplification (NASBA)

Biology; Biochemistry

A method for amplification (up to 10 9 -fold) of a single-stranded RNA template. A reaction mixture contains RNA polymerase, ribonuclease H, reverse transcriptase, nucleoside triphosphates and two ...

hydrogen bond

Biology; Biochemistry

An important cohesive force of biological macromolecules, notably interstrand interactions of bases in the Watson-Crick model of DNA and of amide groups in the α-helix of proteins. The bond is the ...