Molecular biology

Molecular biology

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Molecular biology

vesosome

Biology; Biochemistry

An aggregate of smaller particles packaged into a larger membrane-bound structure. The aggregates are formed by attachment of biotin to individual liposomes or other small particles, then ...

denaturing gradient gel electrophoresis (DGGE technique)

Biology; Biochemistry

A method for detection of single base substitutions in DNA fragments. Putative mutant and normal double-stranded DNA fragments are applied along one edge of an agarose gel slab that contains a ...

SNARE hypothesis

Biology; Biochemistry

A proposal for the mechanism by which membranes, particularly vesicular and Golgi or plasma membranes, fuse during, for instance intracellular transport and secretion. The two membranes contain ...

Descriptive of the interaction of two DNA strands, either single- or double-stranded, in which an oligonucleotide of one strand is twisted into a plait of helices, one around the other, and requires ...

double adaptor method

Biology; Biochemistry

cDNA concatamer sequencing; a method for increasing the efficiency of sequencing cDNA library subclones cDNA fragments are cleaved with a restriction nuclease and randomly religated into larger DNA ...

colour PCR

Biology; Biochemistry

A technique for screening a population for a specific point mutation that uses fluorophore-labelled PCR primers. Three primers are designed for amplification of a restriction fragment that contains ...

coincidence painting (chromosome painting)

Biology; Biochemistry

A method for production and utilization of fluorophore-labelled oligonucleotides to identify very localized regions of chromosomes. The method of degenerate oligonucleotide primed PCR (DOP-PCR) ...