Molecular biology

Molecular biology

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Molecular biology

transduction

Biology; Biochemistry

The conversion of one kind of energy into another, e.g. conversion of the chemical energy of ATP into mechanical energy by muscle contraction; also conversion of a signal as it crosses a barrier, ...

One of the non-enzymic components of a multi- protein signal transduction complex. The complex is assembled upon activation of the receptor or is assembled but dormant until receptor activation. The ...

immunofluorescence

Biology; Biochemistry

The visualization of an antigen in a histological section or on a polyacrylamide slab by allowing it to combine with a fluorophore-tagged antibody. In indirect immunofluorescence the antibody is not ...

primer extension analysis

Biology; Biochemistry

A method for measurement of the amount of a specific mRNA and its length relative to a known sequence. A labelled synthetic primer designed for complementarity to a sequence in the mRNA, usually ...

2-D crystallography

Biology; Biochemistry

A technique for low-resolution (10Â20 Ã) determination of shapes of proteins, especially large, complex proteins that are not amenable to crystallization by conventional techniques. A monomolecular ...

solvent isotope effect

Biology; Biochemistry

The effect on an enzyme's kinetics of inclusion of 2H 2 O; often expressed as D k, the ratio of the reaction rate constant in H 2 O to the rate constant in 2H 2 O. An extension of this approach is a ...

energy balance

Biology; Biochemistry

The thermodynamic necessity that for any organism, the energy intake (nutrients, light for photosynthesis, etc.) must equal the internal heat produced, the work (movement, transport, etc.) and growth ...