Molecular biology glossaries and terms

ligation-mediated PCR

Biology; Biochemistry

A PCR technique, similar to anchored PCR, applied to double-stranded DNA molecules for which the sequence of only one end is known, e.g. in in vivo footprint analysis to detect sites protected by a ...

binary system

Biology; Biochemistry

In transgenic research, an approach to control expression of one transgene by a second, each initially established in its own pedigree. By crossing the two lines, doubly transgenic animals are ...

futile cycle

Biology; Biochemistry

The coupling through common substrate/product pairs of an energy-requiring enzymic reaction with another energy-producing reaction that regenerates one of the products of the first reaction. The net ...

substrate cycle

Biology; Biochemistry

The coupling through common substrate/product pairs of an energy-requiring enzymic reaction with another energy-producing reaction that regenerates one of the products of the first reaction. The net ...

competitive labelling (differential chemical modification)

Biology; Biochemistry

A method for characterization of the microenvironment of an amino acid residue of a protein, e.g. its accessibility to solvent or its pK a ; other residues of the same amino acid serve as internal ...

differential chemical modification

Biology; Biochemistry

A method for characterization of the microenvironment of an amino acid residue of a protein, e.g. its accessibility to solvent or its pK a ; other residues of the same amino acid serve as internal ...

jumping library

Biology; Biochemistry

A tool for the mapping and sequencing of large stretches of a chromosome. Starting at one point in the polynucleotide sequence, a distal point is located without having to first sequence the ...

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