11 Terms
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Molecular biology
Industry: Biology
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Molecular biology
ligation-mediated PCR
Biology; Biochemistry
A PCR technique, similar to anchored PCR, applied to double-stranded DNA molecules for which the sequence of only one end is known, e.g. in in vivo footprint analysis to detect sites protected by a ...
binary system
Biology; Biochemistry
In transgenic research, an approach to control expression of one transgene by a second, each initially established in its own pedigree. By crossing the two lines, doubly transgenic animals are ...
futile cycle
Biology; Biochemistry
The coupling through common substrate/product pairs of an energy-requiring enzymic reaction with another energy-producing reaction that regenerates one of the products of the first reaction. The net ...
substrate cycle
Biology; Biochemistry
The coupling through common substrate/product pairs of an energy-requiring enzymic reaction with another energy-producing reaction that regenerates one of the products of the first reaction. The net ...
competitive labelling (differential chemical modification)
Biology; Biochemistry
A method for characterization of the microenvironment of an amino acid residue of a protein, e.g. its accessibility to solvent or its pK a ; other residues of the same amino acid serve as internal ...
differential chemical modification
Biology; Biochemistry
A method for characterization of the microenvironment of an amino acid residue of a protein, e.g. its accessibility to solvent or its pK a ; other residues of the same amino acid serve as internal ...
jumping library
Biology; Biochemistry
A tool for the mapping and sequencing of large stretches of a chromosome. Starting at one point in the polynucleotide sequence, a distal point is located without having to first sequence the ...
