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Molecular biology
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Molecular biology
NADH
Biology; Biochemistry
NADH (reduced nicotinamide-adenine dinucelotide) acts as an important metabolic electron carrier that carries two electrons as a hydride. It is oxidized to NAD when delivering its pair of electrons ...
cassette mutagenesis
Biology; Biochemistry
A method to determine the limits of tolerance of a protein to amino acid substitution. A cloned gene is mutated by synthesis of short segments of the gene with random base substitutions, insertion of ...
cysteine scanning
Biology; Biochemistry
The use of site-directed mutagenesis to determine, for residues of a complex, usually an uncrystallized protein, their proximity to other residues of the protein, their mileiu (extracellular, ...
cyanobacterium
Biology; Biochemistry
Cyanobacteria (also known as blue-green algae, blue-green bacteria, and Cyanophyta) is a phylum of bacteria that obtain their energy through photosynthesis. The name "cyanobacteria" comes from the ...
transverse relaxation-optimized spectroscopy (TROSY)
Biology; Biochemistry
A variation on correlation spectroscopy (COSY), the two-dimensional NMR technique for correlation of the chemical shifts of two paramagnetic nuclei, which gives much sharper linewidths than COSY and ...
insulator
Biology; Biochemistry
A DNA sequence that contributes to limitation of chromatin activation to distinct segments. In the domain boundary model, the insulator putatively binds nucleoprotein complexes along the chromatin ...
diagonal electrophoresis
Biology; Biochemistry
A method for identification of a particular kind of peptide in a mixture by identical electrophoretic steps, the second at a 908 angle to the first, with a chemical modification introduced between ...
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