Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

A very sensitive method for detection of antigens. The antigen is immobilized in wells of a microtitre plate, exposed to its specific monoclonal antibody, then to a chimaeric protein that consists of ...

primer walking

Biology; Biochemistry

A method for sequencing relatively long pieces of DNA by use of a library of short, and therefore inexpensive, polynucleotides to assemble a primer complementary to a known sequence, initially a ...

abscisic acid

Biology; Biochemistry

Abscisic acid (ABA) is an isoprenoid plant hormone that plays an important part in plant responses to environmental stress and plant pathogens. ABA is produced in terminal buds where it contributes ...

major histocompatibility complex (MHC)

Biology; Biochemistry

The products of genes grouped together on a chromosome that determine whether transplanted tissues will be accepted (if from an individual with the same MHC) or rejected. In the human, they are known ...

immunodiffusion

Biology; Biochemistry

A method for testing the identity of several antigens using an Ouchterlony plate, a glass plate covered with a thin layer of agar on which is incised a hole surrounded by several other holes that ...

base

Biology; Biochemistry

In nucleic acid chemistry, one of the nitrogenous compounds, purines or pyrimidines, that are incorporated into nucleosides, nucleotides or nucleic acids. The most common bases are adenine, cytosine, ...

plus and minus method

Biology; Biochemistry

A variant of the Sanger method of DNA sequencing. A primer is extended by a polymerase to generate a population of newly synthesized deoxyribonucleotides of assorted lengths; the unused dNTPs are ...