Molecular biology

Molecular biology

Industry: Biology

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Molecular biology

open reading frame (ORF)

Biology; Biochemistry

One of three possible reading frames in which an mRNA is potentially translated into protein. In analysis of a DNA sequence, an ORF is characterized by the sequence of nucleotides that when ...

A method to generate a pattern of DNA restriction fragments that is unique to an individual, especially for forensic purposes. DNA from blood, semen or another tissue sample is isolated and cleaved ...

DNA profiling

Biology; Biochemistry

cofactor

Biology; Biochemistry

A compound that is covalently or non-covalently bound to a protein and that participates in the protein's function. In the case of an enzyme, a loosely held cofactor is called a coenzyme, essentially ...

anti conformation

Biology; Biochemistry

In nucleic acid chemistry, the orientation about the glycosidic bond of a nucleoside or nucleotide that places the base away from the sugar moiety; contrasted with the syn conformation, in which the ...

zymography

Biology; Biochemistry

A histological method for localization of an enzymic activity. The tissue or cell preparation overlays a gel that contains a chromophore- or fluorophore-labelled substrate, e.g. casein for a ...

zymogram

Biology; Biochemistry

A slab gel that, after electrophoresis, has been stained to reveal the presence of an enzymic activity, e.g. a gel infused or layered with a protein substrate and subsequently treated with a stain ...