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Molecular biology
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Molecular biology
immunoelectrophoresis
Biology; Biochemistry
A method for analysis of antibody or antigen mixtures. A solution of antibodies is electrophoresed in a gel, then exposed to an antigen solution placed in a trough cut into the gel parallel to the ...
star activity
Biology; Biochemistry
A secondary nucleotide sequence specificity of an endonuclease. After the canonical specificity is established, a library of potential substrates may be created by substituting at each sequence ...
positional candidate approach
Biology; Biochemistry
A strategy for identification and cloning of a new gene; a combination of functional cloning, in which a property of the protein product (e.g. immunogenicity or liganding) is used to scan a cDNA ...
complementary
Biology; Biochemistry
In nucleic acid chemistry, descriptive of the relationship between two polynucleotides that can combine in an antiparallel double helix; the bases of each polynucleotide are in a hydrogen-bonded ...
transesterification
Biology; Biochemistry
The substitution of one alcohol for another in an ester bond, e.g. the displacement of one 3'-hydroxy group of a phosphodiester bond by the 3'-hydroxy group of another nucleotide during the ...
maturase
Biology; Biochemistry
A nuclease involved in RNA processing; it is formed by translation of a fungal mitochondrial mRNA that is produced after a first intron is excised leaving an mRNA in which the open reading frame ...
Abu shunt
Biology; Biochemistry
An alternative pathway in bacteria and higher plants for entry of glutamate into the tricarboxylic acid cycle: glutamate→γ-aminobutyrate (Abu) →succinic acid semialdehyde → succinate.
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