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Molecular biology
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Molecular biology
stringency
Biology; Biochemistry
During nucleic acid hybridization or reassociation, the strictness with which the Watson-Crick base-pairing is required under specified conditions of temperature, pH, salt concentration, etc. ...
treadmilling
Biology; Biochemistry
The dynamic state of microtubules, the addition of subunits at one end, and their removal from the opposite end, which may account for the movement of microtubules in the cell. The nucleation site, ...
thick filament
Biology; Biochemistry
In muscle cells, an aggregate of myosin molecules in which the long, fibrous tails are intertwined in a superhelix that leaves the globular heads as knobs in a helical array over the surface. The ...
dot blotting
Biology; Biochemistry
A method to estimate the concentration of a polynucleotide or protein solution by spotting it on a sheet of nitrocellulose or nylon, hybridizing it with a radiolabelled complementary polynucleotide ...
colony hybridization
Biology; Biochemistry
A technique for screening bacterial colonies for those that contain a desired polynucleotide sequence. A plate is exposed to a labelled oligonucleotide probe with a sequence complementary to part of ...
STAT (signal transducer and activator of transcription)
Biology; Biochemistry
One of a family of proteins that mediate the actions of cytokines and growth factors. When the cytokine or growth factor receptor is occupied, a tyrosine residue of the STAT is phosphorylated and the ...
simulated annealing
Biology; Biochemistry
An energy minimization technique to refine a model structure, e.g. an X-ray crystallographic or nuclear magnetic resonance model, by simulation of heating it to a high temperature to break all ...
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