Molecular biology

Molecular biology

Contributors in Molecular biology

Molecular biology

The creation of an outline of locations of genetic markers (genes or other polynucleotide sequences) within the structures of the chromosomes. The methodology determines the resolution of the map. ...

Kunkel mutagenesis

Biology; Biochemistry

A method to construct a site-specific mutated gene and to select it away from the wild-type. A strain of E. coli with only weak dUTPase activity has high levels of dUTP and consequently incorporates ...

dUTP system mutagenesis

Biology; Biochemistry

prion

Biology; Biochemistry

A proteinaceous infectious particle which causes one of a number of non-inflammatory, slowly-developing degenerative neurological diseases which are characterized by extracellular plaques, notably ...

hydrogen exchange

Biology; Biochemistry

A method for evaluating secondary and tertiary structure of a polypeptide in solution. The amide hydrogens of the polypeptide backbone slowly exchange with water hydrogens, unless they are ...

Koshland model

Biology; Biochemistry

A sequential model for the basis of co-operativity in multimeric binding proteins, originally presented as a description of oxygen binding to each of the haem groups of the haemoglobin tetramer. ...

KNF model

Biology; Biochemistry

A sequential model for the basis of co-operativity in multimeric binding proteins, originally presented as a description of oxygen binding to each of the haem groups of the haemoglobin tetramer. ...