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Molecular biology
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Molecular biology
exon scanning
Biology; Biochemistry
A procedure for approximating sites of alternate splicing. In the PCR amplification of cDNA, a single primer matched to a sequence near one end, 3Â or 5Â, of the coding sequence, is paired with ...
heavy chain
Biology; Biochemistry
One of two or more kinds of polypeptide chain of a heteromultimeric protein, distinguished by molecular mass, e.g. one of the larger polypeptide chains that, when combined with another heavy chain ...
semi-discontinuous
Biology; Biochemistry
Descriptive of the replication of DNA in which the parent strand that is exposed from its 3'-end towards its 5'-end is used as a template for continuous synthesis of a new strand, but the parent ...
coupled-enzyme assay
Biology; Biochemistry
The determination of a substrate or enzyme activity by coupling of one enzymic reaction with another, more easily detectable, reaction. The product of the first reaction is the substrate for the ...
selfish gene
Biology; Biochemistry
A gene which confers a selective advantage upon its own propagation, but not on the survival of the individual. A classic example is Segregation Distorter in D. melanogaster: in heterozygous flies, ...
RNA single-strand conformational polymorphism (rSSCP)
Biology; Biochemistry
A variant of the SSCP technique for detection of mutations in a DNA fragment in which mobility shifts of bands of mutant compared with normal DNA are more pronounced. A phage promoter is added to one ...
pentose phosphate pathway
Biology; Biochemistry
The enzymic reactions that oxidatively convert glucose 6-phosphate into ribulose 5-phosphate and then to intermediates of the glycolytic pathway. The two oxidative reactions are the major source of ...
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